Hamilton Company專注于精密測量設(shè)備，自動化液體處理工作站和樣品管理系統(tǒng)的開發(fā)，制造和定制。Hamilton的流程針對質(zhì)量和靈活性進行了優(yōu)化。無論是具有快速交付時間框架的定制針頭，特殊長度的pH傳感器，還是全面自動化您的檢測工作流程的全面解決方案，相信Hamilton產(chǎn)品將始終滿足您的需求。
Hamilton公司60多年來一直是的制造商，總部位于內(nèi)華達州里諾市; 馬薩諸塞州富蘭克林; 蒂米什瓦拉，羅馬尼亞; 和瑞士的Bonaduz; 和遍布的子公司。
Hamilton為PRP-X100陰離子交換HPLC色譜柱提供了一種快速簡便的方法來制備4.0 mM對羥基苯甲酸/ 2.5％甲醇，pH 8.5流動相。
零件號/ REF | 描述 |
79325 | Eluent Concentrate，PRP-X100陰離子交換，（60 mL瓶裝，每瓶1瓶） |
79335 | Eluent Concentrate，PRP-X100陰離子交換，（60 mL瓶裝，每瓶6瓶） |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-1 | PSDVB* | N/A | 100 Å | 5-85 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-C18 | C18 bonded to PSDVB* | N/A | 100 Å | 5-85 °C | pH 1 – 13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-3 | PSDVB* | N/A | 300 Å | 5-85 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-h5 | Fluorinated PSDVB* | N/A | 300 Å | 5-85 °C | pH 1 – 13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
HxSil C18 | Silica | N/A | 110 Å | 5-60 °C | pH 1.5 – 10. 0-100% aqueous, organic modifier | N/A | 6,000 psi |
HxSil C8 | Silica | N/A | 110 Å | 5-60 °C | pH 1.5 – 10. 0-100% aqueous, organic modifier | N/A | 6,000 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X100 | PSDVB* with Tri- methyl ammonium Exchanger | 0.19meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X110 | PSDVB* with Tri-methyl ammonium Exchanger | 0.11meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X500 | Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride) | 1.6meq/gm | Superficially porous | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-2.0N | 5,000 psi |
PRP-X600 | Poly (di-methyl amido-propyl meth-acrylamide) | 1.6meq/gm | Superficially porous | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
RCX-10 | PSDVB* with Tri-methyl ammonium Exchanger | 0.35meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
RCX-30 | PSDVB* with Tri-methyl ammonium Exchanger | 1.0meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X200 | PSDVB* Sulfonate Exchanger | 35ueq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X400 | PSDVB* Sulfonate Exchanger | 2.5meq/gm | N/A | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-5.0N | 5,000 psi |
PRP-X800 | PSDVB* Itaconate Exchanger | 1.6meq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
HC-40 Calcium | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water | Water | 1,000 psi |
HC-75 Calcium | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
HC-75 Hydrogen | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
HC-75 Lead | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X300 | PSDVB* Sulfonate Exchanger | 0.17meq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C
HPLC柱具有有限的壽命。建議您使用標準定期監(jiān)測色譜柱的保留特性和性能。如果您打算長時間儲存??色譜柱，請使用抑制微生物生長的流動相（含有sodium azide或高濃度甲醇，乙腈等的流動相）。為獲得適性能和色譜柱壽命，請遵循以下建議。
恢復色譜柱性能
下面列出的步驟可能會恢復已經(jīng)污染的色譜柱的性能，但這些過程不會改善損壞或超出其使用壽命的色譜柱的性能。也不能恢復所有固定相。PRP-X500和PRP-X600色譜柱的*支持使得無法通過洗滌程序恢復性能。有關(guān)這些色譜柱的技術(shù)幫助，請HPLC支持。注意：在執(zhí)行恢復程序之前，請務必確保流動相是可混溶的，并且不會發(fā)生沉淀。如有必要，在轉(zhuǎn)換到新的流動相之前，先用合適的中間溶劑清洗色譜柱。應使用至少五個柱體積的中間溶劑。
色譜柱修復程序
按照下面列出的適當修復程序，使用1.0或2.0 mL / min的流速。除非另有規(guī)定。使用每列隨附的性能測試報告中列出的測試混合物和條件評估色譜柱性能。
柱修復程序
Packing Material Name
| Restoration Procedure |
PRP-C18 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-1 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-3 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-h5 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
HxSil C8 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
HxSil C18 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-X100 | Pump approximay 50 mL of methanol with 1% 6 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X200 | Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X300 | Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X400 | Inject Several times with 100 µL of 0.1 M potassium EDTA. Equilibrate with starting phase for ~ 200 column volumes. |
RCX-10 | Flush with 50 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes. |
RCX-30 | Flush with 150 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes. |
HC-40 | Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
HC-75 | Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
| H+ Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
| Pb2+ Flush with 1% lead nitrate at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
零件號/ REF | 描述 |
79641 | 分析型HPLC色譜柱，CUSTOM ORDER（1.0,2.1,4.1,4.6 mm ID） |
79669 | 負離子快 |
79670 | 負離子微 |
79668 | 負離子分辨率 |
79643 | 制備型HPLC色譜柱＃1，定制（21.2,30 mm內(nèi)徑） |
79644 | 制備型HPLC色譜柱＃2，定制（50 mm內(nèi)徑） |
79645 | 制備型HPLC色譜柱＃3，CUSTOM ORDER（100 mm ID） |
79421 | PRP-X10010μm2.1x 150 mm |
79346 | PRP-X10010μm2.1x 250 mm |
79439 | PRP-X10010μm4.1x 100 mm |
79434 | PRP-X10010μm4.1x 150 mm |
79433 | PRP-X10010μm4.1x 250 mm |
79365 | PRP-X10010μm4.1x 50 mm |
79354 | PRP-X10010μm4.6x 150 mm PEEK |
79455 | PRP-X10010μm4.6x 250 mm PEEK |
79353 | PRP-X10012-20μm100Å21.2x 250 mm |
79852 | PRP-X1005μm2.1x 150 mm PEEK |
79190 | PRP-X1005μm2.1x 250 mm |
79538 | PRP-X1005μm4.1x 100 mm |
79812 | PRP-X1005μm4.1x 150 mm |
79810 | PRP-X1005μm4.1x 50 mm |
79174 | PRP-X1005μm4.6x 150 mm PEEK |
79181 | PRP-X1005μm4.6x 250 mm PEEK |
79665 | PRP-X1007μm4.6x 150 mm PEEK |
79642 | 半制備型HPLC色譜柱，CUSTOM ORDER（7.0,7.8,10 mm ID） |
貨號 | 品名 |
79477 | Analytical Guard Cartridge Holder, PEEK |
32908 | Analytical Guard Cartridge Holder, Stainless Steel |
79293 | Analytical Guard Column, PRP-X110 |
79641 | Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID) |
79643 | Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID) |
79644 | Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID) |
79645 | Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID) |
79732 | PRP-X110 7 µm 4.1 x 150 mm |
79734 | PRP-X110 7 µm 4.1 x 250 mm |
79738 | PRP-X110 7 µm 4.6 x 150 mm PEEK |
79729 | PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), PEEK |
79728 | PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel |
79727 | PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK |
79726 | PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel |
79827 | PRP-X110, 7 µm Bulk Resin |
79743 | PRP-X110S 7 µm 2.1 x 100 mm PEEK |
79733 | PRP-X110S 7 µm 4.1 x 150 mm |
79735 | PRP-X110S 7 µm 4.1 x 250 mm |
79741 | PRP-X110S 7 µm 4.6 x 250 mm PEEK |
79642 | Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID) |
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